A primary barrier to structure determination in cryo electron microscopy is preparation of a frozen specimen suitable for high resolution imaging. Problems to overcome include: interactions of molecules with the air-water interface, preferred orientations of the molecules, and movement during irradiation. My project working in the Russo group at the MRC Laboratory of Molecular Biology continues to develop new specimen supports for electron microscopy of biological specimens as well as methods for their manufacture. Our current efforts include the development of methods for high-throughput fabrication of grids which incorporate functionalized graphene as well as approaches for grid surface passivation.